Line for α-cellulose extraction from tree rings

Oak sample - 260 mg. Crumble and cutting of wood, manually

• Reagent: distilled water (175ml), sodium chlorite NaClO₂ (2.5g) and acetic acid (1.7ml) for 1g of sample
• Beakers with samples and solutions are placed in the ultrasonic bath in 70° C
• 6 times, once an hour reagents are added
• Rinse with hot and cold water
• Treatment by 10% NaOH (75ml, ultrasonic bath, 70° C, 45min.)
• Treatment by 17% NaOH (67ml, ultrasonic bath, room temp., 45min.)
• Rinse with distilled water
• Rinse with 1% HCl (20ml)

• Rinse with big amount of distilled water on the hotplate (70° C)
• Drying on the hotplate α-cellulose (70° C)
• One serie for extraction consists of 16 (or 48) samples

• average value of efficiency of α-cellulose extraction is ca 30%